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Related Experiment Video

Updated: Jun 14, 2026

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Published on: May 23, 2010

Live cell microscopy: From image to insight.

Andreas P Cuny, Fabian P Schlottmann¹, Jennifer C Ewald¹

¹Interfaculty Institute of Cell Biology, University of Tuebingen, 72076 Tuebingen, Germany.

Biophysics Reviews

|March 20, 2024

Summary

This summary is machine-generated.

Live-cell microscopy offers direct insights into cellular processes but presents technical challenges. This review guides researchers through experimental setup, image analysis, and data sharing for reproducible live-cell imaging, focusing on yeast.

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Last Updated: Jun 14, 2026

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Live-cell microscopy visualizes cellular behavior and molecular processes, offering direct biological insights.
However, live-cell imaging is technically demanding, requiring expertise across multiple disciplines and careful experimental design.
Artifacts and technical challenges can compromise data quality and interpretation.

Purpose of the Study:

To provide a comprehensive guide for setting up and analyzing live-cell imaging experiments.
To address the interdisciplinary challenges in live-cell microscopy, including hardware selection and image analysis.
To promote reproducibility through recommended reporting and data sharing strategies.

Main Methods:

Literature review and synthesis of best practices in live-cell imaging.
Focus on experimental design considerations from hardware to data analysis.
Discussion of specific challenges and solutions, with a focus on yeast models.

Main Results:

Key considerations for successful live-cell imaging experiments are summarized.
Strategies for mitigating artifacts and ensuring data integrity are presented.
Guidelines for robust reporting and data sharing are proposed to enhance reproducibility.

Conclusions:

Careful planning and execution are crucial for high-quality live-cell microscopy data.
Standardized reporting and data sharing are essential for advancing the field.
This review serves as a practical resource for researchers conducting live-cell imaging studies.