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Updated: Jun 30, 2025

Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
[Skin test (Skin Prick Test) in food allergy]
1Alergólogo e Inmunólogo, Hospital das Clínicas, Universidad de São Paulo. Alergólogo e Inmunólogo, Profesor de Alergología e Inmunología, Universidad de Especialidades Espíritu Santo UEES. Jefe del Departamento de Alergología de Nucleomed, Brasil.todoasma@gmail.com.
Abstract:
Skin tests, also known as prick tests, in food allergies are restricted to IgE-mediated food allergies, with results that barely indicate sensitization to a certain food and do not necessarily suggest food allergy. The clinical context of the patient is the decisive point before performing any type of food allergy skin test; Contextualizing and relating the patient's clinical picture with the immunological mechanism of IgE-mediated allergies will allow a better selection of the allergy test for each case. Positive tests should be interpreted more carefully, and consider that during early childhood it is common that several patients may have negative specific serum IgE tests in the blood for food allergens compared to those performed on the skin, which are positive. Skin testing can be carried out with standardized strata of foods, but they are not always available for all foods and some are very unstable. Skin tests can even be performed with raw foods, using the prick to prick technique to perform the puncture with fresh foods (especially fruits or vegetables) or other products that are not commercially available. The skin prick test is a test where, after having placed the allergenic extract in drops or with fresh food itself, the puncture must be carried out with a standardized lancet.
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