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MoS2-Based Sensor Array for Accurate Identification of Cancer Cells with Ensemble-Modified Aptamers
Ying Xiang1, Jingjing Liu1, Jing Chen1
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, East China Normal University, 500 Dongchuan Road, Shanghai 200241, P. R. China.
This study introduces a novel chemical nose sensor using ensemble-modified aptamer (EMAmer) probes on MoS2 nanosheets for rapid cancer cell identification. The sensor accurately distinguishes between cancer cell types and mixtures, paving the way for advanced cancer diagnostics.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Diagnostics
Background:
- Cancer cell identification remains a challenge, requiring sensitive and specific detection methods.
- Existing diagnostic techniques can be time-consuming and lack the ability to differentiate complex cell mixtures.
- Nanomaterial-based biosensors offer potential for enhanced sensitivity and specificity in disease detection.
Purpose of the Study:
- To develop a novel array-based chemical nose sensor for cancer cell identification.
- To engineer ensemble-modified aptamer (EMAmer) probes for detecting cell surface physicochemical changes.
- To validate the sensor's efficacy in discriminating between various cancer cell types and mixtures.
Main Methods:
- Fabrication of an array-based chemical nose sensor utilizing ensemble-modified aptamer (EMAmer) probes.
- Engineering EMAmer probes with domain-selective functional groups for differential cell interactions.
- Transduction of cellular interactions via competitive adsorption of fluorophore-labeled EMAmer probes on MoS2 nanosheets.
- Utilizing molybdenum disulfide (MoS2) nanosheets as a substrate for probe immobilization.
Main Results:
- The MoS2-EMAmer sensor array successfully discriminated among six types of cancer cells and their mixtures.
- Rapid identification was achieved within 60 minutes for cell concentrations as low as 10^4 cells.
- The sensor demonstrated a high accuracy of 94.4% in identifying blinded unknown cancer cell samples.
- Differential interactions between EMAmer probes and cancer cells were effectively transduced.
Conclusions:
- The developed MoS2-EMAmer sensing platform provides a rapid and effective method for cancer cell identification.
- This technology shows significant promise for advancing cancer diagnostics and personalized medicine.
- The sensor's ability to differentiate complex mixtures highlights its potential for clinical applications.
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