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Updated: Jun 30, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
External quality control survey on identification of microorganisms using matrix-assisted laser desorption/ionization
Kazuyuki Sogawa1, Azumi Fujinaga2, Hajime Okumura3
1Department of Biochemistry, School of Life and Environmental Science, Azabu University.
Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry( MALDI-TOF MS) is a bacterial typing tool that was approved as a medical device in 2011. However, external accuracy control examination of bacterial typing using mass spectrometry is still only performed on a small scale. In this study, E. faecium and S. maltophilia were selected and tested according to established procedures using Score Values at 228 institutions. The Score Values for MALDI Biotyper were 2.43±0.08 for E. faecium and 2.38±0.08 for S. maltophilia; and those for VITEK MS/PRIME were 99.9±0.0 for E. faecium and S. maltophilia. These results suggest that it is useful to evaluate external accuracy control with Score Values using the procedures we have developed.
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