Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems

Area of Science:

• Biomedical Engineering
• Developmental Biology
• Microscopy

Background:

• Live imaging of multicellular systems presents challenges due to their growth over weeks from single cells to complex tissues or organisms.
• Bridging spatiotemporal scales is crucial for understanding developmental processes and tissue dynamics.

Purpose of the Study:

• To present an advanced light-sheet microscope designed for live imaging of large specimens at single-cell resolution.
• To overcome the limitations of current imaging techniques for dynamic, large-scale biological samples.

Main Methods:

• Development of an open-top dual-view and dual-illumination light-sheet microscope.
• Integration of a specific objective configuration with a customizable multiwell mounting system.
• Implementation of high-throughput multiposition imaging capabilities.

Main Results:

• The microscope successfully imaged diverse large specimens, including mature intestinal organoids and gastruloids.
• Demonstrated capability to acquire quantitative single-cell information from these complex biological models.
• The dual-view and dual-illumination setup facilitated comprehensive imaging of large samples.

Conclusions:

• The developed light-sheet microscope effectively addresses the need for high-resolution live imaging of large multicellular systems.
• This technology provides a powerful tool for quantitative analysis of cellular dynamics in complex biological structures.
• Enables new avenues for research in developmental biology and tissue engineering.