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Compact Quantum Dots for Single-molecule Imaging
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Blinking Carbon Dots as a Super-resolution Imaging Probe.

Jayanta Dolai1, Prakash Joshi2, Partha Pratim Mondal2,3

  • 1School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India.

ACS Applied Materials & Interfaces
|March 21, 2024
PubMed
Summary
This summary is machine-generated.

This study presents fluorescent carbon dots (CDs) as super-resolution imaging probes for single-molecule localization microscopy (SMLM). Different CDs offer distinct blinking properties, enabling tailored applications in bioimaging.

Keywords:
carbon dotfluorescence blinkingfluorescent probenanoparticlesuper-resolution imaging

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Single-molecule localization microscopy (SMLM) requires advanced probes for nanoscale imaging.
  • Developing probes with specific photophysical properties is critical for SMLM.

Purpose of the Study:

  • To demonstrate fluorescent carbon dots (CDs) as SMLM probes.
  • To investigate the relationship between CD composition, blinking behavior, and imaging applications.

Main Methods:

  • Synthesis of red and green emissive carbon dots from a common precursor.
  • Characterization of photophysical properties, including blinking behavior.
  • Application of CDs as probes in SMLM for super-resolution imaging.

Main Results:

  • Two distinct fluorescent carbon dots (red and green) were developed for SMLM.
  • CDs exhibited spontaneous blinking with high photon counts and varying duty cycles.
  • Red CDs (lower blinking cycle) are suitable for quantitative analysis; green CDs (higher blinking cycle) for high-resolution imaging.
  • Localization precision of 20 nm and resolution of 60 nm were achieved.
  • Differences in blinking linked to chemical composition and trap states.

Conclusions:

  • Carbon dots can be engineered as versatile probes for SMLM.
  • Tailoring CD properties allows for diverse bioimaging applications.
  • This work advances SMLM probe development for nanoscale visualization.