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Photo-Excited Dyes: Emerging Technique Against Tau Protein Aggregation
Subashchandrabose Chinnathambi1,2,3, Tushar Dubey4,5
1Neurobiology Group, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, India. subashneuro@nimhans.ac.in.
Methods in Molecular Biology (Clifton, N.J.)
|March 21, 2024
Summary
Rose bengal shows potential for Alzheimer's disease treatment by inhibiting Tau aggregation. Photo-excited rose bengal effectively disaggregated Tau fibrils with low toxicity, suggesting a novel therapeutic strategy.
Area of Science:
- Neuroscience
- Biochemistry
- Pharmacology
Background:
- Tau aggregates are a key pathological marker in Alzheimer's disease (AD).
- Targeting Tau aggregation is a promising therapeutic strategy for AD.
- Photo-excited molecules have demonstrated therapeutic efficacy in various diseases.
Purpose of the Study:
- To investigate the effect of rose bengal (RB) on Tau aggregation.
- To evaluate the potential of photo-excited rose bengal (PE-RB) in disaggregating Tau fibrils.
Main Methods:
- In vitro studies utilizing electron microscopy.
- Thioflavin S (ThS) fluorescence assays.
- Circular dichroism (CD) spectroscopy.
Main Results:
- Rose bengal attenuated Tau aggregation under in vitro conditions.
- Photo-excited rose bengal effectively disaggregated mature Tau fibrils.
- Both classical and photo-excited rose bengal exhibited low cellular toxicity.
Conclusions:
- Rose bengal demonstrates potential as a therapeutic agent for Alzheimer's disease.
- The study highlights the efficacy of photo-excited rose bengal in combating Tau pathology.
- Further investigation into rose bengal for AD treatment is warranted.
