Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

13.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.3K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Hematopoiesis01:21

Hematopoiesis

5.3K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SIRT2 antagonizes MOF function during mitotic entry.

Science advances·2026
Same author

The roles of the acetyltransferase domains of the chromatin regulators KAT6A and KAT6B in vivo.

Development (Cambridge, England)·2026
Same author

The benchmarking and application of tag-degraders in vivo to validate therapeutic targets.

Nature communications·2026
Same author

KAT6A is essential for developmental control gene expression in neural stem and progenitor cells.

PLoS genetics·2026
Same author

The GFI1-FOXO1 axis regulates NK cell maturation and function.

Nature communications·2026
Same author

Valorization of avocado (cv. Hass) waste powder in industrial-scale sourdough "ciabatta" bread production: Impact on microbial dynamics, quality attributes, and phenolic bioaccessibility.

Food research international (Ottawa, Ont.)·2026

Related Experiment Video

Updated: Jun 30, 2025

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

840

The histone acetyltransferase KAT6B is required for hematopoietic stem cell development and function.

Maria I Bergamasco1, Nishika Ranathunga1, Waruni Abeysekera1

  • 1The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Melbourne, VIC 3052, Australia.

Stem Cell Reports
|March 22, 2024
PubMed
Summary

The histone acetyltransferase KAT6B supports fetal hematopoietic stem cells. KAT6B and KAT6A work together to promote blood stem cell development and function, relevant for cancer therapy research.

Keywords:
KAT6AKAT6Bchromatinhematopoiesishistone acetyltransferasestem cellstransplantation

More Related Videos

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

21.3K

Related Experiment Videos

Last Updated: Jun 30, 2025

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

840
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

21.3K

Area of Science:

  • Hematology
  • Epigenetics
  • Cancer Biology

Background:

  • The histone lysine acetyltransferase KAT6B is implicated in hematological malignancies.
  • Chromosomal translocations targeting KAT6B are associated with poor prognosis in cancer patients.

Purpose of the Study:

  • To investigate the role of KAT6B in the hematopoietic system using mouse models.
  • To determine the specific functions of KAT6B in hematopoietic stem cell (HSC) maintenance and differentiation.

Main Methods:

  • Germline deletion and overexpression of Kat6b in mice.
  • Analysis of histone acetylation patterns, specifically H3K9 acetylation.
  • Assessment of hematopoietic reconstitution after transplantation in compound heterozygotes of Kat6b and Kat6a.

Main Results:

  • KAT6B sustains the fetal hematopoietic stem cell pool without affecting viability or differentiation.
  • KAT6B is essential for histone H3 lysine 9 (H3K9) acetylation.
  • Compound heterozygosity of Kat6b and Kat6a impairs hematopoietic reconstitution.
  • KAT6B and KAT6A cooperatively regulate the transcription of key hematopoiesis genes.

Conclusions:

  • KAT6B plays a critical role in maintaining fetal HSCs.
  • KAT6B and KAT6A act synergistically to promote HSC development, function, and gene transcription.
  • These findings have implications for cancer therapeutics targeting KAT6A/B inhibitors.