Related Experiment Video

Updated: Jun 30, 2025

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

6.4K

A fungal core effector exploits the OsPUX8B.2-OsCDC48-6 module to suppress plant immunity

Xuetao Shi1,2, Xin Xie1, Yuanwen Guo1

  • 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Nature Communications
|March 23, 2024
PubMed

Insights

Magnaporthe oryzae

Area of Science:

  • Plant-pathogen interactions
  • Molecular plant-pathology
  • Protein quality control mechanisms

Background:

  • Ubiquitin regulatory X (UBX) domain proteins are cofactors of Cell Division Cycle 48 (CDC48) involved in protein quality control.
  • The role of UBX proteins in host-microbe interactions is not well understood.
  • Magnaporthe oryzae is a fungal pathogen that causes rice blast disease.

Purpose of the Study:

  • To investigate the role of UBX-containing proteins in rice immunity against Magnaporthe oryzae.
  • To elucidate the mechanism by which the fungal effector MoNLE1 interacts with rice proteins.

Main Methods:

  • Yeast two-hybrid assays to identify protein interactions.
  • Co-immunoprecipitation to confirm protein binding.
  • Quantitative real-time PCR to analyze gene expression.
  • Confocal microscopy to observe protein localization.

Main Results:

  • MoNLE1, a Magnaporthe oryzae effector, suppresses rice immunity by targeting OsPUX8B.2.
  • OsPUX8B.2's UBX domain is crucial for its interaction with OsATG8 and OsCDC48-6, regulating its stability via the 26S proteasome.
  • OsPUX8B.2 and OsCDC48-6 enhance rice immunity, while OsBHT negatively regulates defense against blast fungus.
  • MoNLE1 induces OsPUX8B.2 degradation and disrupts its interaction with OsBHT.

Conclusions:

  • MoNLE1 virulence relies on disrupting the OsPUX8B.2-OsCDC48-6 complex, leading to immune suppression.
  • Engineering intracellular proteins resistant to MoNLE1 could confer broad and durable blast resistance in plants.

More Related Videos

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

13.2K
Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins05:38

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

11.4K

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K
Plant Cell Wall02:43

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.5K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
23.7K