Related Experiment Video

Updated: Aug 17, 2026

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

Development and characterization of nuclear microsatellite markers for the African walnut Coula edulis Baill

Narcisse Guy Kamdem^1,2, Saskia Sergeant³, Camille Vercruysse³

¹Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, Brussels, B-1050, Belgium. guynarcissekamdem@yahoo.fr.

Molecular Biology Reports

|March 23, 2024

Abstract

Premise Of The Study:

Coula edulis Baill (Coulaceae) is a common tree species in the Guineo-Congolian forests producing an edible fruit known as African walnut, which is an important food and income resource for rural populations. However, the species suffers from a deficit of natural regeneration. We developed here nuclear microsatellite markers for C. edulis to be able to study the genetic structure of its natural populations and gene flow.

Methods And Results:

A genomic library was obtained using the Illumina platform, and 21 polymorphic microsatellite loci were developed. The polymorphic microsatellites displayed eight to 22 alleles per locus (average: 14.2), with a mean expected heterozygosity ranging from 0.33 to 0.72 in five populations from Central and West Africa.

Conclusions:

The high polymorphism of the nuclear microsatellite markers developed makes them useful to investigate gene flow and the organization of genetic diversity in C. edulis, and to assess whether particular genetic resources require conservation efforts.

Keywords:

Coula edulis African rainforest Gene flow Genetic diversity Microsatellites

More Related Videos

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

Related Concept Videos

Seed Structure and Early Development of the Sporophyte

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.

Fruit Development, Structure, and Function

Fruit Development, Structure, and Function

Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.

Articles linked to this work by shared authors, journal, and citation graph.

Macroevolutionary dynamics and predictors of tropical rain forest plant diversity.

Science advances·2026

Phylogenetic biome conservatism underlies the evolution of forest palaeoendemic legume trees in tropical Africa.

Proceedings. Biological sciences·2026

Seed dispersal and predation of the African walnut tree, Coula edulis Baill. (Coulaceae): contrasting fates in intact and hunted rainforests.

BMC plant biology·2026

Managed rainforests support higher carbon density and sequestration in the Congo Basin.

Nature communications·2026

High selfing rate and inbreeding depression correlate with past range expansion in an endangered African timber species Pericopsis elata.

Heredity·2026

Evidence of high genetic differentiation driven by limited gene flow in a lower canopy African tropical rainforest tree species, Coula edulis Baill. (Coulaceae).

BMC ecology and evolution·2025

See all related articles