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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
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Mobile genetic element-based gene editing and genome engineering: Recent advances and applications
Jaeseong Hwang1, Dae-Yeol Ye2, Gyoo Yeol Jung3
1School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.
Biotechnology Advances
|March 23, 2024
Summary
Mobile genetic elements (MGEs) are versatile genome engineering tools, offering solutions for genetic manipulation. This review details MGEs like transposons and their applications in advancing genetic engineering.
Area of Science:
- Genomics and Molecular Biology
- Mobile Genetic Elements (MGEs) in Genome Engineering
Background:
- Genome engineering has advanced various scientific fields, including therapeutics and agriculture.
- Existing genome engineering toolkits effectively modify organism genomes.
- A gap exists in comprehensive reviews of genome engineering technologies utilizing mobile genetic elements (MGEs).
Purpose of the Study:
- To provide a comprehensive review of mobile genetic elements (MGEs) as powerful genome engineering tools.
- To highlight the versatility and efficiency of MGEs in addressing genome engineering challenges.
- To update the scientific community on MGE-based genome engineering technologies.
Main Methods:
- Review of literature on genome engineering technologies based on MGEs.
- Analysis of MGE components, mechanisms, and features.
- Focus on cellular applications of MGE-based tools.
Main Results:
- MGEs, including DNA transposons, retrotransposons, retrons, and CRISPR-associated transposons, offer significant advantages.
- Key advantages include broad host range, genome-wide mutagenesis, large DNA integration, multiplexing, and in situ single-stranded DNA generation.
- MGEs provide efficient solutions for various genome engineering applications.
Conclusions:
- The integration of genome engineering with MGEs shows immense potential for overcoming challenges in genetic manipulation.
- MGE-based technologies promise to revolutionize the engineering and understanding of diverse organism genomes.
- This review offers insights into the evolving landscape and future prospects of MGE-based genome engineering.
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