Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

9.4K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.4K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.6K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DHX36 is a regulatory switch in the interferon-mediated antiviral response.

Science advances·2026
Same author

The Zuo1 C-terminal domain stabilizes DNA guanosine quadruplex (G4) structures located on Chromosome IX in Saccharomyces cerevisiae.

Nucleic acids research·2025
Same author

Poly (ADP-ribose) polymerase in yeasts: characterization and involvement in telomere maintenance.

Nucleic acids research·2025
Same author

The Shu complex interacts with the replicative helicase to prevent mutations and aberrant recombination.

The EMBO journal·2025
Same author

Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.

Cell host & microbe·2024
Same author

Phosphatidylinositol 4-Kinase III Alpha Governs Cytoskeletal Organization for Invasiveness of Liver Cancer Cells.

Gastroenterology·2024

Related Experiment Video

Updated: Jun 30, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K

In-gel staining methods of G4 DNA and RNA structures.

Philipp Schult1, Katrin Paeschke1

  • 1Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, Germany.

Methods in Enzymology
|March 23, 2024
PubMed
Summary

This study explores using light-up probes to detect G-quadruplexes (G4) in vitro. This accessible method visualizes G4 structures in RNA using native gels and fluorescent probes.

Keywords:
DetectionG-quadruplexG4 specific dye

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

610

Related Experiment Videos

Last Updated: Jun 30, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

610

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Nucleic Acid Chemistry

Background:

  • G-quadruplexes (G4) are crucial nucleic acid structures involved in cellular processes.
  • In vivo detection of dynamic G4s is challenging, necessitating in vitro validation.
  • Various methods exist for G4 detection, each with limitations.

Purpose of the Study:

  • To present an accessible in vitro method for G-quadruplex detection.
  • To detail the procedure of using light-up probes for G4 visualization.
  • To discuss the advantages and limitations of this technique.

Main Methods:

  • Utilizing native gel electrophoresis for G4 structure separation.
  • Employing light-up probes that fluoresce upon binding to G4s.
  • Focusing on the application of the NMM probe with RNA G4s.

Main Results:

  • Light-up probes offer a quick and accessible method for G4 detection.
  • The NMM probe demonstrates utility in visualizing RNA G4 structures.
  • The method allows for G4 detection without specialized equipment.

Conclusions:

  • Light-up probes are versatile tools for G4 visualization in vitro.
  • This technique provides an accessible alternative for initial G4 structure confirmation.
  • Understanding the advantages and limitations is key for effective application.