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Uncommon Salmonella Infantis Variants with Incomplete Antigenic Formula in the Poultry Food Chain, Italy
Uncommon Salmonella Infantis variants with incomplete antigen formulas were identified. Molecular methods revealed these variants were genetically similar to wild-type Salmonella Infantis, despite phenotypic differences.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Salmonella Infantis is an important foodborne pathogen.
- Antigenic characterization is crucial for Salmonella serotyping.
- Phenotypic and genotypic methods can sometimes yield discordant results.
Purpose of the Study:
- To investigate uncommon Salmonella Infantis variants.
- To compare phenotypic and molecular characteristics of these variants.
- To determine the genetic relatedness of variants to wild-type Salmonella Infantis.
Main Methods:
- Phenotypic analysis of colony morphology and antigenic formula.
- Molecular serotyping techniques.
- Antimicrobial resistance profiling.
- Detection of the usg gene.
- Whole-genome sequencing (WGS).
Main Results:
- Uncommon Salmonella Infantis variants exhibited incomplete antigenic formulas phenotypically.
- Molecular serotyping differentiated these variants from wild-type strains.
- Most variants formed rough colonies but shared antimicrobial resistances and the usg gene with wild-type strains.
- Whole-genome sequencing could not distinguish these variants from wild-type Salmonella Infantis.
Conclusions:
- Phenotypic and molecular methods can reveal discrepancies in Salmonella Infantis characterization.
- Despite phenotypic variations, uncommon variants share key genetic and resistance traits with wild-type strains.
- Whole-genome sequencing provides a robust tool for identifying true genetic identity, even with atypical presentations.
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