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Neferine Pretreatment Attenuates Isoproterenol-Induced Cardiac Injury Through Modulation of Oxidative Stress,

Xiaoqian Sun1, Yongwen Gu2, Xinghua Liu3

  • 1Cardiovascular Medicine Department, Xi'an Gaoxin Hospital, Xi'an, 710000, China.

Applied Biochemistry and Biotechnology
|March 25, 2024
PubMed

Insights

Neferine, a natural compound, protects against heart attacks (myocardial infarctions) by reducing cardiac dysfunction, inflammation, and oxidative stress in rats. This study highlights neferine

Area of Science:

  • Cardiology
  • Pharmacology
  • Biochemistry

Background:

  • Cardiovascular diseases (CVDs), including heart attacks (myocardial infarctions or MIs), are a leading global cause of mortality.
  • Neferine, an alkaloid from Nelumbo nucifera seeds, exhibits potential therapeutic properties.
  • Investigating natural compounds like neferine is crucial for developing novel treatments for cardiac dysfunction.

Purpose of the Study:

  • To evaluate the cardioprotective effects of neferine against isoproterenol (ISO)-induced myocardial infarction in a rat model.
  • To elucidate the underlying molecular mechanisms of neferine's action.

Main Methods:

  • Myocardial infarction was induced in rats using isoproterenol (ISO).
  • Rats were pretreated with varying doses of neferine (10 or 20 mg/kg) for 28 days.
  • Cardiac function, lipid profiles, histopathology, oxidative stress, inflammation, and apoptosis markers were assessed. Molecular signaling pathways (Nrf2/Keap1/ARE, TLR4/NF-κB/MAPK) were analyzed. Molecular docking was performed using CB-Dock-2.

Main Results:

  • Neferine pretreatment significantly protected against ISO-induced cardiac dysfunction, improving lipid profiles and cardiac functional markers.
  • Histopathological analysis revealed that neferine attenuated myocardial fibrosis, collagen deposition, and cellular damage.
  • Neferine inhibited oxidative stress, inflammation, and apoptosis by modulating key signaling pathways, including stimulating Nrf2/Keap1/ARE and inhibiting TLR4/NF-κB/MAPK.
  • Molecular docking indicated high binding affinity of neferine with relevant protein targets.

Conclusions:

  • Neferine demonstrates significant cardioprotective effects against ISO-induced heart damage in rats.
  • The protective mechanisms involve the modulation of oxidative stress, inflammation, and apoptosis via specific signaling pathways.
  • Neferine holds promise as a potential therapeutic agent for preventing or treating myocardial infarction.

Keywords:
Cardiac injuryInflammationMolecular dockingMyocardial infarctionNeferineOxidative stress

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