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Related Experiment Video

Updated: Jun 29, 2025

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Liquid Glycan Array.

Chih-Lan Lin¹, Eric J Carpenter¹, Taoran Li¹

¹Department of Chemistry, University of Alberta, Edmonton, AB, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2024

Summary

This summary is machine-generated.

This study introduces liquid glycan arrays (LiGA) using M13 bacteriophage as a nanoscale carrier. These LiGAs enable precise control over glycan density for studying lectin binding and identifying optimal glycoconjugates for cellular and in vivo applications.

Keywords:

Lectin binding Liquid glycan arrays M13 phage Multivalent Phage-displayed glycans

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Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

Area of Science:

Nanotechnology
Glycobiology
Bioconjugation

Background:

M13 bacteriophage is a versatile nanoscale carrier for molecular modification.
Chemical conjugation allows for the attachment of various molecules to the phage surface.
Understanding glycan-phage interactions is crucial for developing targeted delivery systems.

Purpose of the Study:

To develop a novel method for creating multivalent glycoconjugates using the M13 phage platform.
To establish liquid glycan arrays (LiGA) for studying glycan-lectin interactions.
To investigate the impact of glycan density on binding avidity to lectins in vitro and in vivo.

Main Methods:

M13 phage pVIII protein was acylated with dibenzocyclooctyne for reaction with azido glycans.
Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) spectrometry was used to quantify glycan density (30-1500 copies/phage).
Liquid glycan arrays (LiGA) were prepared with encoded glycan structure and density.

Main Results:

Successfully generated multivalent glycoconjugates with controlled glycan densities on M13 phage.
Demonstrated the utility of LiGA for validating glycan-lectin binding properties.
Showcased the ability of LiGA to identify optimal glycoconjugates for lectin binding to cells and in vivo.

Conclusions:

M13 phage serves as an effective platform for creating tunable multivalent glycan structures.
LiGA technology provides a powerful tool for glycan-based research and development.
This approach has potential applications in diagnostics, therapeutics, and understanding biological recognition processes.