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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 29, 2025

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Published on: November 2, 2012

Cytometry in High-Containment Laboratories.

Melanie Cohen¹, Julie Laux¹, Iyadh Douagi²

¹Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2024

Summary

This summary is machine-generated.

High-containment laboratories need advanced cell analysis technologies like cytometry for infectious disease research. Integrating cytometry and emerging tech enhances global preparedness for new pathogens.

Keywords:

Aerosols Biosafety CYTOF Cell analysis Cell sorting Cytometry Emerging pathogens Flow cytometry High containment Infectious disease Infectious sample Multiomics Public health

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Area of Science:

* Cell biology
* Infectious disease research
* Biosafety

Background:

* Emerging pathogens necessitate advanced high-containment laboratories globally.
* Cytometry is a crucial cell analysis technology for infectious disease research, vaccine development, and biosafety.
* Integrating cytometry into high-containment settings presents unique challenges and opportunities.

Approach:

* Review current applications of cytometry in infectious disease, vaccine research, and biosafety.
* Discuss specific considerations for cytometry in high-containment labs, including biosafety and sample containment.
* Explore emerging technologies like multi-omics integration, automation, and artificial intelligence.

Key Points:

* Cytometry applications in infectious disease research, vaccine development, and biosafety are expanding.
* Biosafety requirements and sample containment are critical for cytometry in high-containment labs.
* Emerging technologies like automation, AI, and multi-omics integration offer significant advancements.

Conclusions:

* A framework is proposed to accelerate the adoption of advanced technologies in high-containment research.
* Enhancing high-containment laboratory capabilities is vital for global preparedness against emerging diseases.
* Integrating cytometry and novel technologies will improve response to future pandemics.