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The MultiBac Protein Complex Production Platform at the EMBL
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Split Proteins and Reassembly Modules for Biological Applications
Jieun Bae1, Jonghoon Kim2, Jongdoo Choi1
1Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan, 46241, Republic of Korea.
Chembiochem : a European Journal of Chemical Biology
|March 26, 2024
Summary
Split systems, modular biological tools, are revolutionizing research. This review details their advancements in gene regulation, protein interactions, and biosensors, highlighting progress over the last decade.
Area of Science:
- Molecular Biology
- Biotechnology
- Synthetic Biology
Background:
- Split systems are modular biological tools enabling controlled processes.
- They are crucial for applications in gene regulation, protein interaction studies, and biosensor development.
Purpose of the Study:
- To review the significant progress in split systems over the last decade.
- To provide a comprehensive perspective on the evolution and applications of split systems in biological research.
Main Methods:
- Review of traditional split proteins (e.g., GFP, luciferase, inteins).
- Exploration of advancements in split technologies (e.g., Cas proteins, base editors).
- Examination of reassembly modules and their diverse applications.
Main Results:
- Split systems have evolved significantly, incorporating new technologies.
- Reassembly modules demonstrate broad utility across various research fields.
- Recent advancements enhance control over biological processes.
Conclusions:
- Split systems are indispensable tools in modern biological research.
- Their applications span from fundamental gene regulation to therapeutic innovation.
- Continued evolution promises further breakthroughs in biotechnology.
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