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Mn2+-Mediated Modulation of PfAgo Activity for Biosensing
Ruijie Fu1,2, Jinjie Hou1,2, Zexiang Wang1,3
1College of Biosystems Engineering and Food Science, Zhejiang University, Zhejiang, 310058, China.
Advanced Healthcare Materials
|March 26, 2024
Summary
This study introduces a novel biosensing method using manganese ions to enhance Argonaute (Ago) enzyme activity. This approach enables sensitive detection of various analytes, offering a versatile tool for bioanalysis.
Area of Science:
- Biochemistry
- Biotechnology
- Analytical Chemistry
Background:
- Argonaute (Ago) enzymes are valuable tools in biosensing due to their programmability and sensitivity.
- Current Ago-based biosensing strategies often rely on phosphorylated guide DNA, limiting versatility.
Purpose of the Study:
- To explore manganese ion (Mn2+)-enhanced cleavage activity of Ago for improved biosensing.
- To develop a versatile biosensing platform using Pyrococcus furiosus Ago (PfAgo) and variable valence Mn ions.
Main Methods:
- Investigated Mn2+-enhanced cleavage activity of PfAgo.
- Utilized MnO2 nanoflowers to convert Mn ions with different valence states.
- Developed a PfAgo-based immunoassay for target detection.
Main Results:
- Achieved sensitive detection of ascorbic acid (2.5 nmol L-1), alkaline phosphatase (0.009 U L-1), and arsenic (0.4 ng mL-1).
- Demonstrated the conversion of Mn ions with different valence states to regulate PfAgo activity.
- Successfully developed a PfAgo-based immunoassay for diverse target detection.
Conclusions:
- Manganese ions significantly enhance PfAgo cleavage activity for biosensing.
- The developed method offers a versatile and sensitive platform for detecting multiple analytes.
- This approach broadens the application of PfAgo-based sensors in bioanalytical and biomedical fields.
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