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Advances in recombinant protease production: current state and perspectives
Xiufang Liu1,2, Mulin Lian1,2, Mouming Zhao1,2
1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
World Journal of Microbiology & Biotechnology
|March 27, 2024
Summary
Recombinant protease production is crucial for meeting industrial demands. This review explores microbial expression systems and engineering techniques to enhance protease yield and application compatibility.
Area of Science:
- Biochemistry and Molecular Biology
- Microbial Biotechnology
Background:
- Proteases are vital enzymes with broad applications in food, biotech, and medicine.
- Native protease hosts often limit high-level expression and downstream processing.
- Recombinant production offers a solution to overcome these limitations.
Purpose of the Study:
- To review the current status of protease production in microbial systems.
- To highlight advancements in protease engineering over the last five years.
- To provide insights into the future of recombinant protease research.
Main Methods:
- Overview of prokaryotic (Bacillus spp.) and eukaryotic (yeast) expression systems for protease production.
- Discussion of recent protease engineering strategies, including rational design and directed evolution.
- Synthesis of current trends and key findings in recombinant protease research.
Main Results:
- Bacillus species are leading prokaryotic hosts for protease expression.
- Yeasts are widely utilized as eukaryotic expression systems.
- Protease engineering has seen significant progress through rational design and directed evolution.
Conclusions:
- Recombinant protease production in microbial systems is essential for industrial needs.
- Advancements in expression systems and engineering techniques are driving progress.
- Future research holds promise for further optimizing protease production and applications.
Keywords:
Expression systemsGenetic engineeringHigh-throughput screeningProtease engineeringRecombinant proteasesMore Related Videos
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