Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films

Area of Science:

• Biomaterials Science
• Wound Healing
• Polymer Chemistry

Background:

• Chronic skin wound treatment faces challenges requiring advanced biomaterials.
• Existing treatments often lack optimal mechanical and hydration properties.
• Chitosan and hydrolyzed collagen are promising components for wound dressings.

Purpose of the Study:

• To formulate and characterize chitosan (Cs)/hydrolyzed collagen (HC) films.
• To evaluate the mechanical and hydration performance of these films.
• To identify optimal formulations for chronic skin wound treatment.

Main Methods:

• Solvent casting method used to create eight film formulations with varying plasticizers (glycerin, PEG1500).
• Physico-chemical, mechanical, and hydration properties were characterized.
• Full factorial design statistically analyzed the effects of components and plasticizers.
• In vitro cell viability assay using HaCaT cells.

Main Results:

• Solid-state characterization confirmed the hybrid nature of Cs/HC films, suggesting electrostatic interactions.
• Films with high HC concentration (2% w/v) and glycerin or glycerin/PEG1500 exhibited superior mechanical and swelling properties.
• Statistical analysis identified key factors influencing film behavior.
• No significant difference in HaCaT cell viability was observed, indicating good biocompatibility.

Conclusions:

• Chitosan/hydrolyzed collagen films, particularly those with high HC and glycerin, are promising biomaterials for chronic skin wound treatment.
• The developed films possess favorable mechanical and hydration characteristics.
• These biomaterials show potential for biomedical applications in wound healing due to their biocompatibility.