Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Overview of Exosomes01:36

Overview of Exosomes

2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 3D Nanofiltration Array for Direct Plasma EV Purification with Label-Free SERS Detection toward Accurate Clinical Breast Cancer Staging.

ACS sensors·2025
Same author

Real-Time Three-Dimensional Imaging of Cell-Substrate Adhesion Structures Based on Plasmonic Scattering Microscopy.

ACS sensors·2025
Same author

A Wearable Dual-Modal Patch for Rapid Pre-Hospital Diagnosis of Acute Myocardial Infarction.

ACS nano·2025
Same author

Apoptotic bodies encapsulating Ti<sub>2</sub>N nanosheets for synergistic chemo-photothermal therapy.

Nanotechnology·2024
Same author

Chemically Powered Nanomotors with Magnetically Responsive Function for Targeted Delivery of Exosomes.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

A MXene Hydrogel-Based Versatile Microrobot for Controllable Water Pollution Management.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024

Related Experiment Video

Updated: Jun 29, 2025

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.8K

Highly Accurate Profiling of Exosome Phenotypes Using Super-resolution Tricolor Fluorescence Co-localization.

Jinxiu Wei1, Kai Zhu1, Tingyu Wang1

  • 1Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing, Jiangsu 210096, China.

ACS Nano
|March 27, 2024
PubMed
Summary

A novel SR-TFC method accurately distinguishes exosomes from different cell lines by combining super-resolution imaging and pixel counting, overcoming nonspecific adsorption for reliable cancer diagnosis.

Keywords:
exosomesmulticolor fluorescence co-localizationphenotypespixel countingsuper-resolution fluorescence imaging

More Related Videos

An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

30.8K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.4K

Related Experiment Videos

Last Updated: Jun 29, 2025

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.8K
An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

30.8K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.4K

Area of Science:

  • Biomarker Discovery
  • Nanotechnology
  • Cancer Research

Background:

  • Exosomes are rich in proteomic information, serving as potential noninvasive biomarkers for early disease diagnosis, particularly in cancer.
  • Accurately distinguishing exosomes from different cell lines is challenging, and current fluorescence immunoassays are affected by unavoidable nonspecific adsorption.
  • Existing methods to reduce nonspecific adsorption are insufficient, highlighting the need for more effective solutions.

Purpose of the Study:

  • To develop a convenient and highly reliable method (SR-TFC) to eliminate artifacts from nonspecific adsorption in exosome detection.
  • To demonstrate the capability of SR-TFC for profiling exosomal membrane proteins and identifying breast cancer subpopulations.
  • To establish a foundation for multiplex phenotypic analysis of exosomes for precise cancer diagnosis.

Main Methods:

  • Development of the SR-TFC method, integrating tricolor fluorescence labeling, tricolor super-resolution imaging, and a novel pixel counting technique (CFPP) for artifact removal.
  • Implementation of the CFPP method using MATLAB to eliminate nonspecific binding sites at the single-pixel level.
  • Application of SR-TFC for multiplex phenotypic analysis by labeling three specific proteins to obtain 3D phenotypic information of exosomes.

Main Results:

  • The SR-TFC method effectively removes artifacts caused by nonspecific adsorption, significantly improving detection reliability.
  • Profiling of exosomal membrane proteins and identification of breast cancer subpopulations were successfully demonstrated.
  • Accurate identification of breast cancer subtypes was achieved using super-resolution images of clinically relevant exosomal proteins.

Conclusions:

  • The SR-TFC method offers a convenient and highly reliable approach to overcome nonspecific adsorption in exosome detection.
  • This technique enables accurate classification of breast cancer subtypes based on exosomal protein profiles.
  • SR-TFC holds significant potential for clinical cancer diagnosis and the advancement of precision medicine, with applicability to other cancer types by selecting different biomarkers.