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Comparison of different freezing methods for micro-volume semen.

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For micro-volume semen freezing, the static liquid nitrogen vapor cooling (FLNV) method is superior to direct rapid freezing (RFLN) for volumes under 50 uL. Larger volumes (over 100 uL) showed better post-thaw outcomes.

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Area of Science:

  • Reproductive Medicine
  • Cryobiology
  • Andrology

Background:

  • Micro-volume semen cryopreservation is vital for fertility preservation in cancer patients and those undergoing surgeries affecting fertility.
  • Challenges remain in optimizing frozen-thawed semen efficiency.

Purpose of the Study:

  • To determine the optimal semen freezing method based on sample volume.
  • To compare the efficacy of two distinct cryopreservation techniques.

Main Methods:

  • Investigated frozen-thawed outcomes of semen samples ranging from 20 uL to 1 mL.
  • Utilized two freezing methods: static liquid nitrogen vapor cooling (FLNV) and direct rapid freezing in liquid nitrogen (RFLN).
  • Assessed sperm vitality, progressive motility, and DNA fragmentation index post-thaw.

Main Results:

  • Semen volumes exceeding 100 uL demonstrated superior post-thaw results compared to volumes ≤ 50 uL.
  • The FLNV method showed higher cryopreservation efficiency for semen volumes under 50 uL.

Conclusions:

  • The FLNV technique is more effective than RFLN for small-volume semen cryopreservation.
  • Optimal freezing strategies should consider semen volume for improved fertility preservation outcomes.