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Updated: Jun 29, 2025

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

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PIP4K2B Protein Regulation by NSD1 in HPV-Negative Head and Neck Squamous Cell Carcinoma

Iuliia Topchu1,2, Igor Bychkov1,3, Ekaterina Roshchina1

  • 1Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Division of Hematology/Oncology, Northwestern University, Chicago, IL 60611, USA.

Cancers
|March 28, 2024
PubMed

Insights

This study identifies PIP4K2B as a key downstream target of NSD1 in head and neck squamous cell carcinoma (HNSCC). NSD1 regulates PIP4K2B, impacting cancer cell growth, particularly in laryngeal HNSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Head and neck squamous cell carcinoma (HNSCC) has a persistent low survival rate.
  • The histone methyltransferase NSD1 is implicated as a potential oncogenic factor in HNSCC.

Purpose of the Study:

  • To investigate the role of NSD1 in HNSCC pathogenesis.
  • To identify downstream targets of NSD1 and their impact on cancer cell growth.

Main Methods:

  • Reverse Phase Protein Array (RPPA) analysis.
  • Validation studies to confirm molecular interactions.
  • In vitro cell growth assays.

Main Results:

  • PIP4K2B identified as a key downstream target of NSD1 in HNSCC.
  • NSD1 positively regulates PIP4K2B transcription via H3K36me2.
  • PIP4K2B depletion downregulates the mTOR pathway, inhibiting cell growth.
  • PIP4K2B overexpression rescues growth in laryngeal HNSCC cells.

Conclusions:

  • PIP4K2B is a novel NSD1-dependent protein in HNSCC.
  • PIP4K2B plays a context-dependent role in HNSCC cell survival.
  • PIP4K2B is a potential therapeutic target for laryngeal cancer.
Keywords:
NSD1PIP4KPIP4K2Bhead and neck cancer squamous cell carcinoma (HNSCC)mTORC1

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