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Related Experiment Videos

Human kidney kallikrein: cDNA cloning and sequence analysis.

A R Baker, J Shine

    DNA (Mary Ann Liebert, Inc.)
    |December 1, 1985
    PubMed
    Summary

    Researchers elucidated the primary structure of human kidney kallikrein using molecular cloning. This finding confirms the kidney as a significant source of kallikrein, revealing gene polymorphisms.

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    Patents and literature.

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    Area of Science:

    • Biochemistry
    • Genetics
    • Molecular Biology

    Background:

    • Kallikreins are serine proteases involved in various physiological processes.
    • The primary structure of human kidney kallikrein was not fully characterized.
    • Mammalian glandular kallikreins share structural homologies.

    Purpose of the Study:

    • To elucidate the primary structure of human kidney kallikrein.
    • To identify the kidney as a major source of kallikrein.
    • To analyze the structural homology and genetic variations of human kidney kallikrein.

    Main Methods:

    • Molecular cloning and cDNA sequence analysis were employed.
    • A human kidney cDNA library was screened for kallikrein sequences.
    • Southern blot analysis was used to investigate related sequences in human DNA.

    Main Results:

    • The primary structure of human kidney kallikrein was determined.
    • Human kidney kallikrein showed significant homology to mammalian glandular kallikreins.
    • The kidney was confirmed as a major source of kallikrein.
    • Several polymorphisms in human kallikrein genes were identified.

    Conclusions:

    • The human kidney is a significant source of kallikrein.
    • Conserved catalytic residues and specificity-determining residues were identified.
    • Human kallikrein genes exhibit polymorphisms, suggesting genetic diversity.

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