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Related Experiment Video

Updated: May 11, 2026

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies

Published on: April 11, 2012

In Vitro Simulated Hemoperfusion on Seraph^®-100 as a Promising Strategy to Counteract Sepsis.

Antonio Lacquaniti¹, Antonella Smeriglio², Susanna Campo¹

¹Nephrology and Dialysis Unit, Department of Internal Medicine, Papardo Hospital, 98158 Messina, Italy.

|March 28, 2024

Summary

Seraph®-100 effectively reduced bacterial load in simulated hemoperfusion, adsorbing 10⁸ CFU/mL of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Maximum sequestration occurred within 30 minutes, demonstrating its potential in blood purification for sepsis treatment.

Keywords:

Seraph®-100 acute kidney injury adsorption renal replacement therapy sepsis

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Published on: June 28, 2018

Area of Science:

Biomedical Engineering
Infectious Diseases
Critical Care Medicine

Background:

Sepsis involves hyper-inflammation and immune system over-activation.
Blood purification offers a potential therapeutic strategy for sepsis.
Seraph®-100 is a device investigated for blood purification efficacy.

Purpose of the Study:

To investigate the binding efficacy of Seraph®-100 against common sepsis-causing bacteria.
To evaluate bacterial reduction during simulated hemoperfusion treatment.
To analyze bacterial sequestration kinetics and distribution within the filter.

Main Methods:

Simulated hemoperfusion with 10⁸ CFU/mL of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli.
Fluorescence-activated cell sorting (FACS) for bacterial load reduction assessment.
Kinetic analysis, bacterial cultures, and scanning electron microscopy (SEM) for detailed evaluation.

Main Results:

FACS revealed a 78.77% total bacterial load reduction by the end of treatment.
Maximum bacterial sequestration by the Seraph®-100 filter occurred within the first 30 minutes.
Kinetic analysis showed a 99% reduction rate for all tested bacteria, with S. aureus exhibiting a lower growth rate, indicating greater affinity.

Conclusions:

Seraph®-100 demonstrated significant adsorption of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa during in vitro simulated hemoperfusion.
The filter effectively reduced bacterial load, with sequestration primarily occurring in the initial phase of treatment.
SEM analysis confirmed bacterial adsorption throughout the filter, with approximately 50% concentrated in the filter head.