Fine Wrinkle Improvement through Bioactive Materials That Modulate EDAR and BNC2 Gene Expression

Area of Science:

• Dermatology
• Genetics
• Cosmetic Science

Background:

• Skin aging is a complex process influenced by intrinsic and extrinsic factors.
• There is a growing demand for highly effective anti-aging ingredients to combat wrinkles.
• Existing wrinkle-improving materials often have limitations in efficacy.

Purpose of the Study:

• To investigate the mechanisms of wrinkle-related genes identified in genome-wide association studies (GWASs).
• To identify novel ingredients that regulate these specific genes.
• To develop and validate an effective anti-wrinkle formula superior to current standards.

Main Methods:

• Selected two candidate genes, EDAR and BNC2, linked to periorbural wrinkles.
• Conducted in vitro experiments on human skin cell lines (keratinocytes and fibroblasts) to study gene function.
• Identified and validated ingredients regulating EDAR and BNC2 expression using cell line models.
• Developed a novel anti-wrinkle formula and conducted human clinical trials.

Main Results:

• Confirmed the role of EDAR and BNC2 in skin aging mechanisms.
• Identified specific ingredients that effectively modulate the expression of these genes.
• The developed formula demonstrated enhanced dermal collagen production in 3D skin models.
• Clinical trials showed the formula significantly improved under-eye wrinkles compared to retinol over 8 weeks.
• The formula exhibited no adverse side effects like skin irritation.

Conclusions:

• The study successfully identified novel targets and ingredients for wrinkle improvement.
• A new anti-wrinkle formula with superior efficacy and safety to retinol was developed.
• This research offers a promising, well-tolerated solution for combating skin aging and wrinkles.