Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ciliary protein ARL13b detected on RBCs as a potential indicative biomarker of vaso-occlusive crisis and disease severity in SCD: a retrospective pilot study.

Journal of sickle cell disease·2026

Same author

Acylglycerol Kinase 2-mediated Inhibition of Sirtuin 2 Restores AMPK/AKT/mTOR Signaling Balance in Podocytes: A Pharmacological Strategy for Diabetic Nephropathy.

Iranian journal of pharmaceutical research : IJPR·2026

Same author

Dual TYK2/JAK1 Inhibition by Brepocitinib Reprograms Synoviocyte Pathobiology: Mechanistic Insights Into Targeted Therapy for Rheumatoid Arthritis.

Iranian journal of pharmaceutical research : IJPR·2026

Same author

Guide for Selecting Experimental Models to Study Dietary Fat Absorption.

Nutrients·2025

Same author

Citronellol Reduces Sepsis-Induced Renal Inflammation via AP-1/NF-κB/TNF-α Pathway.

Biomolecules·2025

Same author

L-carvone alleviates lipopolysaccharide-Induced acute kidney injury via modulating MyD88-dependent and independent signaling pathways in Mice.

Molecular biology reports·2025

Search research articles

Related Experiment Video

Updated: Jun 29, 2025

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

Limonene Exerts Anti-Inflammatory Effect on LPS-Induced Jejunal Injury in Mice by Inhibiting NF-κB/AP-1 Pathway.

Sarmed H Kathem¹, Yasameen Sh Nasrawi², Shihab H Mutlag¹

¹Pharmacology and Toxicology Department, College of Pharmacy, University of Baghdad, Baghdad 92618, Iraq.

|March 28, 2024

Summary

Limonene protects against sepsis-induced gut injury by reducing inflammation and preserving kidney function. This natural compound shows potential for maintaining gastrointestinal health.

Keywords:

IL-1β IRF3 TLR4 TNF-α iNOS intestinal injury monoterpenes

More Related Videos

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

Related Experiment Videos

Last Updated: Jun 29, 2025

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

Area of Science:

Gastroenterology
Pharmacology
Toxicology

Background:

Sepsis can compromise the intestinal barrier, with lipopolysaccharide (LPS) exacerbating this damage.
Intestinal barrier dysfunction contributes to inflammation and systemic complications.
Natural compounds are being investigated for protective effects against sepsis-induced injuries.

Purpose of the Study:

To investigate the protective effects of limonene against LPS-induced jejunal injury in a mouse model.
To evaluate limonene's impact on renal function and inflammatory markers during sepsis.

Main Methods:

Mice were administered oral limonene (100 and 200 mg/kg) before LPS injection.
Murine Sepsis Score (MSS) assessed sepsis severity.
Serum urea and creatinine levels measured renal function.
Pro-inflammatory cytokines (TNF-α, IL-1β, COX-2), signaling pathways (TLR4/NF-κB/AP-1, IRF3), and oxidative stress markers (Nrf2) were analyzed.

Main Results:

LPS injection led to renal function deterioration, indicated by increased serum urea and creatinine.
Limonene pretreatment significantly mitigated the decline in renal function.
Limonene reduced pro-inflammatory cytokines and modulated oxidative stress via Nrf2 activation.
Limonene suppressed TLR4/NF-κB/AP-1 signaling pathways.

Conclusions:

Limonene demonstrates protective effects against LPS-induced jejunal injury and renal dysfunction in mice.
Limonene exhibits anti-inflammatory and antioxidant properties relevant to sepsis management.
Limonene is a promising therapeutic candidate for preserving gastrointestinal health during sepsis.