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Correction: Dinescu et al. Graphene Oxide Enhances Chitosan-Based 3D Scaffold Properties for Bone Tissue Engineering. <i>Int. J. Mol. Sci.</i> 2019, <i>20</i>, 5077.

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Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid.

Georgian Alin Toader1,2, Valentin Titus Grigorean3,4, Mariana Ionita1,2,5

  • 1Faculty of Medical Engineering, National University of Science and Technology Politehnica Bucharest, Gheorghe Polizu 1-7, 011061 Bucharest, Romania.

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A novel hybrid nitrocellulose membrane modified with amino-polyethylene glycol functionalized reduced graphene oxide efficiently extracts short DNA strands. This material shows enhanced performance in specific ionic solutions, improving oligonucleotide recovery.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Efficient extraction of short oligonucleotides is crucial for molecular biology applications.
  • Existing methods often face challenges with recovery rates and specificity in complex solutions.
  • Nitrocellulose (NC) membranes are widely used but can be modified to enhance their functionality.

Purpose of the Study:

  • To synthesize and characterize a novel hybrid material for oligonucleotide extraction.
  • To evaluate the performance of the hybrid material in extracting short single-stranded DNA (ssDNA).
  • To investigate the influence of ionic species on the extraction efficiency.

Main Methods:

  • Synthesis of a hybrid membrane by non-covalently modifying nitrocellulose (NC) with amino-polyethylene glycol functionalized reduced graphene oxide (NH2-PEG-rGO).
  • Characterization using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM).
  • Evaluation of membrane wettability via contact angle measurements.
  • Performance testing for ssDNA extraction from solutions containing MnCl2 and MgCl2.

Main Results:

  • FTIR confirmed successful modification, and SEM revealed morphological changes with a smoother surface.
  • The hybrid membrane exhibited hydrophobic behavior (contact angle > 90°), unlike the hydrophilic NC membrane.
  • Optimal ssDNA extraction occurred in MgCl2, yielding approximately 610 pg (13% recovery), significantly higher than the pristine NC membrane (286 pg, 6% recovery).

Conclusions:

  • The developed NC/NH2-PEG-rGO hybrid membrane is an effective material for oligonucleotide extraction.
  • The material demonstrates improved performance in the presence of specific divalent cations like MgCl2.
  • This hybrid material holds promise for enhancing nucleic acid purification techniques.