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Exploring the miRNAs Profile in Dark-Cutting Beef.

Laura González-Blanco1,2, Luis J Royo1,3, Yolanda Diñeiro1,2

  • 1Área de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, Spain.

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Summary
This summary is machine-generated.

Stressors deplete muscle glycogen, causing dark, firm, and dry (DFD) beef. Differential microRNA (miRNA) expression in muscle at 24 hours post mortem indicates early beef quality defects, linked to cellular stress responses.

Keywords:
DFD beefMiRNAmeat qualityoxidative stressskeletal muscle

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Area of Science:

  • Animal Science
  • Molecular Biology
  • Food Science

Background:

  • Animal production stressors can deplete muscle glycogen stores, leading to elevated pH post mortem (pH24).
  • This metabolic alteration results in meat quality defects, specifically dark, firm, and dry (DFD) beef.
  • Small non-coding RNAs, or microRNAs (miRNAs), are implicated in regulating cellular processes affected by stress.

Purpose of the Study:

  • To investigate if differential miRNA expression in Longissimus thoracis et l. muscle at 24 h post mortem can predict beef quality defects.
  • To identify specific miRNAs associated with the development of dark, firm, and dry (DFD) beef.

Main Methods:

  • Total RNA extraction and comprehensive miRNAome sequencing were performed on muscle tissue.
  • Differential miRNA expression analysis was conducted between DFD and control beef samples.
  • Quantitative RT-PCR validated specific miRNA expressions, and Western blotting analyzed target protein levels.

Main Results:

  • Twelve miRNAs were significantly upregulated in DFD beef compared to control beef (p < 0.001).
  • Upregulated miRNAs are primarily involved in cellular responses to oxidative stress and apoptosis.
  • DFD beef showed high 70 kDa heat shock protein and low Caspase-9 expression, indicating apoptosis downregulation.

Conclusions:

  • Differential miRNA abundance at 24 h post mortem serves as an early indicator of beef quality defects.
  • miRNAs play a crucial role in regulating muscle cell stress responses during the early post mortem period.
  • Altered miRNA expression impacts muscle-to-meat conversion, leading to undesirable meat quality characteristics like DFD beef.