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Updated: Jun 29, 2025
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Published on: April 23, 2014
Generation of human-induced pluripotent stem cell line from PBMC of healthy donor using integration-free Sendai virus
Jaganmay Sarkar1, Shreya Dhepe1, Amrita Shivalkar1
1Department of Integrated Drug Discovery and Development, C113 Yashraj Biotechnology Ltd, TTC Industrial Area MIDC, Pawnae, Navi Mumbai 400705, Maharastra, India.
Abstract:
We developed a well-characterized human induced pluripotent stem cell (iPSC) line obtained from healthy individuals' peripheral blood mononuclear cells (PBMC). The PBMCs were primed and reprogrammed using a non-integrating sendai viral vector, and the iPSC lines demonstrated complete differentiation capacity. This line, YBLi004-A, is available and registered in the human pluripotent stem cell registry. The line's legitimacy was validated using pluripotent marker expression, in vitro differentiation into three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis. This iPSC line could be used as a healthy control for studies involving disease-specific-iPSCs, e.g. drug toxicity and efficacy testing.
