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Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth
Anja Lindis Dahl1, Martin Barfred Friis1, Hanne Wiese Hallberg1
1Department of Clinical Microbiology, Copenhagen University Hospital - Herlev and Gentofte, Herlev, Denmark.
Diagnostic Microbiology and Infectious Disease
|March 28, 2024
Abstract:
Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

