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In vitro osteoclastogenesis assessment using murine myeloid-derived suppressor cells.

Kyu Hwan Kwack1, Lixia Zhang2, Keith L Kirkwood3

  • 1Department of Oral Biology, University at Buffalo, Buffalo, NY, United States; Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea.

Methods in Cell Biology
|March 30, 2024
PubMed
Summary
This summary is machine-generated.

Myeloid-derived suppressor cells (MDSCs) can differentiate into osteoclasts, contributing to bone destruction in cancer and inflammatory diseases. This study details methods to isolate and evaluate these cells for broader research applications.

Keywords:
Monocytic myeloid-derived suppressor cellsMyeloid-derived suppressor cellsOsteoclastOsteoclastogenesis

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Area of Science:

  • Immunology
  • Cell Biology
  • Pathology

Background:

  • Myeloid-derived suppressor cells (MDSCs) are known for their immunosuppressive roles in cancer immunology.
  • A novel function of MDSCs as osteoclast precursors is emerging, particularly concerning monocytic-MDSCs (M-MDSCs).
  • M-MDSCs share a myeloid lineage with macrophages, which are established osteoclast progenitors.

Purpose of the Study:

  • To detail a technique for evaluating osteoclasts in vitro.
  • To present specific methods for isolating and identifying M-MDSCs.
  • To provide an adaptable protocol for M-MDSC isolation from various pathological conditions.

Main Methods:

  • In vitro osteoclast evaluation assays.
  • Isolation of monocytic-MDSCs (M-MDSCs).
  • Identification techniques for M-MDSCs.

Main Results:

  • Demonstration of M-MDSC differentiation into functional osteoclasts.
  • Establishment of a reliable protocol for M-MDSC isolation.
  • Validation of M-MDSC's role in bone destruction beyond cancer.

Conclusions:

  • M-MDSCs serve as osteoclast precursors, contributing to bone pathology in cancer and inflammatory conditions like obesity and osteoarthritis.
  • The presented protocol facilitates the isolation and evaluation of M-MDSCs across diverse pathological contexts.
  • This research opens avenues for understanding and potentially targeting MDSC-mediated bone destruction.