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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

721
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
721

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Living in Syn: T Cell Antigen Identification Based on Synapse Sequencing.

Venkata Krishna Kanth Makani1,2,3, Alok V Joglekar1,2,3

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Researchers developed Tsyn-seq, a new platform for discovering T cell epitopes. This method sequences T cell receptor-peptide-Human Leukocyte Antigen interactions to screen for known epitopes genome-wide.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genomics

Background:

  • T cell responses are crucial in immunity and disease.
  • T cells recognize peptide antigens presented by Human Leukocyte Antigens (HLA) via T cell receptors (TCRs).
  • Despite millions of identified TCRs, few have characterized epitopes.

Purpose of the Study:

  • To introduce Tsyn-seq, a novel cell-based platform for antigen discovery.
  • To enable genome-wide screening of T cell epitopes.

Main Methods:

  • Tsyn-seq utilizes sequencing of T cell receptor-peptide-HLA-induced synapses.
  • This method facilitates the identification of TCR-epitope pairings.

Main Results:

  • The study reports the development and application of the Tsyn-seq platform.
  • Tsyn-seq enables high-throughput screening for T cell epitopes.

Conclusions:

  • Tsyn-seq represents a significant advancement in T cell antigen discovery.
  • This platform can accelerate the identification of TCRs specific to known epitopes.