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Updated: Jun 29, 2025
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Published on: January 19, 2018
Imaging Neuropeptide Release from Drosophila Clock and Motor Neurons
Edwin S Levitan1, Dinara Bulgari2, Markus K Klose2
1Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA elevitan@pitt.edu.
Abstract:
Electrophysiological studies of synaptic function do not robustly report release of neuropeptides and neurotrophins. These limitations have been overcome with the presynaptic expression of optical release reporters based on green fluorescent protein and fluorogen-activating protein. Here we describe how to image neuropeptide release in Drosophila at the neuromuscular junction and in the adult brain.
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