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Updated: May 12, 2026

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Electrical Impedance Spectroscopy Quantifies Skin Barrier Function in Organotypic In Vitro Epidermis Models.

N J M van den Brink¹, F Pardow^1,2, L D Meesters^1,2

¹Department of Dermatology, Radboudumc, Nijmegen, The Netherlands.

Biorxiv : the Preprint Server for Biology

|April 2, 2024

Summary

This summary is machine-generated.

Electrical impedance spectroscopy (EIS) non-invasively measures 3D human epidermal equivalent (HEE) barrier function. EIS quantifies epidermal development, defects, and repair, offering insights into skin health and disease.

Keywords:

Human epidermal equivalents TEER electrical impedance spectroscopy epidermal barrier in vitro epidermis model reconstructed human epidermis skin barrier function

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Area of Science:

Dermatology
Toxicology
Biophysics

Background:

3D human epidermal equivalents (HEEs) are advanced models for pre-clinical research in dermatology and toxicology.
Assessing epidermal barrier function is crucial for understanding skin health and disease.
Non-invasive methods are needed for continuous monitoring of HEEs.

Approach:

Developed a custom electrical impedance spectroscopy (EIS) setup with a 12-electrode pair lid for 24-transwell systems.
Performed serial EIS measurements over seven days on HEEs, assessing impact on morphology and barrier function.
Correlated impedance spectra features (EIS ^diff and EIS ^SC) with specific epidermal characteristics like differentiation and stratum corneum thickness.

Key Points:

EIS measurements did not affect HEE morphology and showed consistent trends over time.
EIS ^diff correlated with keratinocyte terminal differentiation, while EIS ^SC correlated with stratum corneum thickness.
Genetic modifications (CRISPR/Cas9) and cytokine exposure confirmed EIS ^diff's sensitivity to differentiation and barrier defects.

Conclusions:

EIS offers a non-invasive, quantitative method for assessing HEE epidermal barrier function.
This technique can consecutively monitor barrier development, defects, and repair in HEE models.
EIS provides objective readouts for evaluating therapeutic molecule efficacy in restoring barrier function.