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Related Experiment Video

Updated: Jun 29, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

Light-gated Integrator for Highlighting Kinase Activity in Living Cells.

Wei Lin¹, Abhishek Phatarphekar¹, Yanghao Zhong¹

¹Department of Pharmacology, University of California San Diego, La Jolla, CA, USA.

Biorxiv : the Preprint Server for Biology

|April 2, 2024

Summary

Researchers developed a new tool called KINACT to record transient kinase activity as permanent fluorescent marks. This technology allows for better monitoring of kinase signaling in cells and aids in discovering new drugs.

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Last Updated: Jun 29, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

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Published on: October 4, 2024

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Area of Science:

Molecular Biology
Cell Signaling
Biotechnology

Background:

Protein kinases are crucial regulators of biological processes and disease.
Existing fluorescent biosensors visualize kinase activity in real-time but lack recording capabilities.
There is a need for tools to record transient kinase signals for further analysis and manipulation.

Conclusions:

KINACT is a powerful tool for recording transient kinase signaling events.
This technology enhances understanding of kinase signaling in biological and disease processes.
KINACT facilitates drug discovery and mechanistic studies of signaling pathways.