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Related Concept Videos

Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

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DNA Framework-Programmed Nanoscale Enzyme Assemblies.

Nan Cao1,2, Ruiyan Guo1,3, Ping Song4

  • 1School of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Nano Letters
|April 2, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D nanoscale enzyme assembly using tetrahedral DNA frameworks (TDFs). This innovation enhances enzyme cascade activity and enables sensitive detection of metabolism biomarkers creatinine and creatine.

Keywords:
3D Multienzyme AssembliesActivity EnhancementDNA FrameworkEnzyme CascadeNanoscale Phase Separation

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Multienzyme assemblies are vital for cellular processes.
  • Precise spatial control of enzyme complexes in vitro is challenging.
  • Existing methods lack the ability to program 3D enzyme configurations.

Purpose of the Study:

  • To develop a nanoscale 3D enzyme assembly with controlled spatial configuration.
  • To investigate the impact of 3D assembly on enzyme cascade activity.
  • To demonstrate the application of 3D enzyme assemblies in biomarker detection.

Main Methods:

  • Utilized a tetrahedral DNA framework (TDF) to create 3D nanoscale enzyme assemblies.
  • Incorporated glucose oxidase (GOx)/horseradish peroxidase (HRP) in two-enzyme systems.
  • Incorporated amylglucosidase (AGO)/GOx/HRP in three-enzyme systems.
  • Evaluated catalytic activity and efficiency compared to free enzymes and low-dimensional structures.
  • Applied the assemblies for the detection of creatinine and creatine.

Main Results:

  • Achieved precise replication of spatial topological configuration and edge-to-edge distances.
  • Demonstrated significant enhancements in cascade catalytic activity: ~5.9-fold for two-enzyme systems and ~7.7-fold for three-enzyme systems compared to homogeneous mixtures.
  • Successfully detected metabolism biomarkers creatinine and creatine with low limits of detection, high sensitivity, and a broad detection range.

Conclusions:

  • The developed 3D nanoscale enzyme assemblies offer a novel platform for programming enzyme activity in vitro.
  • These assemblies significantly enhance enzyme cascade efficiency.
  • The technology shows promise for sensitive and reliable biomarker detection applications.