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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Related Experiment Video

Updated: Jun 29, 2025

Cryogenic Liquid Jets for High Repetition Rate Discovery Science
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A hermetic on-cryostat helium source for low temperature experiments.

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A novel cryogenic helium source cell, sealed in-situ, provides a stable liquid helium supply for experiments. This method prevents temperature instabilities, improving quantum fluid and device research.

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Area of Science:

  • Cryogenics
  • Quantum Fluids
  • Superconducting Devices

Background:

  • Cryogenic experiments require a stable and controlled supply of helium.
  • Traditional helium transfer methods can introduce temperature instabilities.
  • Unwanted thermal effects hinder precise measurements in quantum systems.

Purpose of the Study:

  • To develop an in-situ helium source cell for cryogenic applications.
  • To eliminate temperature fluctuations caused by helium transfer tubes.
  • To enhance the stability of cryogenic experimental environments.

Main Methods:

  • A hermetically sealed helium source cell is integrated onto a cryostat's cold plate.
  • Helium gas is introduced at room temperature and cold-welded for sealing.
  • A superconducting resonator monitors helium condensation and collection.

Main Results:

  • The in-situ helium source successfully condensed helium at low temperatures.
  • Temperature instabilities were mitigated by eliminating the helium transfer tube.
  • The superconducting resonator effectively detected liquid helium accumulation.

Conclusions:

  • The developed on-cryostat helium source offers a stable and reliable method for cryogenic experiments.
  • This technique is suitable for studying quantum fluid properties.
  • It can also be applied to improve thermalization of quantum devices.