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On-chip light sheet illumination for nanoparticle tracking in microfluidic channels.

Théo Travers1, Gaétan Delhaye1, Martinus H V Werts2

  • 1Laboratoire MOLTECH-Anjou, UMR CNRS 6200, Univ Angers, SFR MATRIX, 2 Bd Lavoisier, 49000 Angers, France. matthieu.loumaigne@univ-angers.fr.

Analytical Methods : Advancing Methods and Applications
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Summary

This study presents an inexpensive method using an optical fiber in a microfluidic chip for nanoparticle tracking. This technique enhances signal-to-noise ratio for accurate nanoparticle sizing and dark-field imaging.

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Area of Science:

  • Microfluidics
  • Optical Engineering
  • Nanotechnology

Background:

  • Accurate nanoparticle tracking and sizing are crucial in various scientific fields.
  • Traditional methods for nanoparticle analysis can be complex and costly.
  • Integrating efficient illumination into microfluidic systems presents technical challenges.

Purpose of the Study:

  • To develop a simple, inexpensive, and efficient method for light sheet illumination in microfluidic chips.
  • To enable dark-field microscopic tracking and sizing of nanoparticles.
  • To improve the signal-to-noise ratio for nanoparticle analysis.

Main Methods:

  • Integration of an optical fiber as a cylindrical lens within a polydimethylsiloxane (PDMS) microfluidic chip.
  • Utilizing the optical fiber to shape and homogenize the light sheet illumination.
  • Employing calculations, numerical simulations, and experimental measurements to optimize the light sheet configuration.
  • Dark-field microscopy for nanoparticle tracking and sizing.

Main Results:

  • The optical fiber insertion effectively smoothed PDMS microchannel walls and ensured perpendicular illumination.
  • An optimal configuration achieved a slowly converging light sheet with near-uniform axial thickness (12 μm).
  • A six-fold increase in signal-to-noise ratio was observed compared to systems without the integrated optical fiber lens.
  • Successful tracking and sizing of 80 nm and 50 nm gold nanoparticles were demonstrated.

Conclusions:

  • The presented method offers a cost-effective and straightforward approach for advanced nanoparticle analysis.
  • Integrating optical fibers as micro-optical components significantly enhances light sheet illumination quality in microfluidics.
  • This technique provides a robust platform for precise nanoparticle characterization using dark-field microscopy.