Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cumulative Spectroscopic Detection for Taylor Dispersion Analysis of Nanoparticles.

Analytical chemistry·2026
Same author

Chemistry and light: an adventure into probing, understanding and controlling events at the molecular scale.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2024
Same author

On-chip light sheet illumination for nanoparticle tracking in microfluidic channels.

Analytical methods : advancing methods and applications·2024
Same author

Determination of photoluminescence quantum yields in dilute solution using non-monochromatic excitation light.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2022
Same author

Brownian Motion and Large Electric Polarizabilities Facilitate Dielectrophoretic Capture of Sub-200 nm Gold Nanoparticles in Water.

Chemphyschem : a European journal of chemical physics and physical chemistry·2019
Same author

Spectroscopic and Hydrodynamic Characterisation of DNA-Linked Gold Nanoparticle Dimers in Solution using Two-Photon Photoluminescence.

Chemphyschem : a European journal of chemical physics and physical chemistry·2018
Same journal

A tetrahedral probe constellation approach for measuring canonical momentum in self-organized laboratory plasma.

The Review of scientific instruments·2026
Same journal

High-precision and short duration operating time dispersion in a fast mechanical switch driven by an ultrasonic motor: Modeling, prediction, and compensation.

The Review of scientific instruments·2026
Same journal

Cluster assisted soft-landing hub (CLASH): An instrument for surface desorption and deposition using a pulsed cluster ion source.

The Review of scientific instruments·2026
Same journal

Influence of pre-ionization parameters on multi-channel discharge characteristics of field-distortion switch gaps.

The Review of scientific instruments·2026
Same journal

A Joule-Thomson low-temperature scanning tunneling microscope with vector magnet and rotatable scanning head.

The Review of scientific instruments·2026
Same journal

Fiber-optic triggering of a two-stage high-current linear transformer driver with laser energy below 100 μJ.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Multi-tau pulsed illumination differential dynamic microscopy with 80 μs resolution.

Emmanuel Schaub1, Martinus H V Werts2

  • 1University of Rennes, CNRS, Institut de Physique de Rennes-UMR 6251, F-35000 Rennes, France.

The Review of Scientific Instruments
|June 1, 2026
PubMed
Summary
This summary is machine-generated.

Multi-tau pulsed illumination differential dynamic microscopy (MTPI-DDM) significantly enhances time resolution for studying microscale dynamics. This pulsed method improves short-timescale measurements in soft matter systems.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Related Experiment Videos

Last Updated: Jun 2, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Area of Science:

  • Soft Condensed Matter Physics
  • Materials Science
  • Optical Microscopy

Background:

  • Differential dynamic microscopy (DDM) is a powerful technique for analyzing microscale dynamics in soft condensed matter.
  • Conventional DDM methods face limitations in achieving high time resolution, especially at the fastest timescales.
  • Enhancing the temporal resolution of DDM is crucial for a comprehensive understanding of dynamic processes.

Purpose of the Study:

  • Introduce multi-tau pulsed illumination DDM (MTPI-DDM) to significantly improve DDM's time resolution.
  • Develop a cost-effective DDM setup using readily available components.
  • Extend the capability of DDM to probe faster dynamic phenomena.

Main Methods:

  • Utilized a multi-tau timing sequence with a geometric progression of time lags for high sampling density at short timescales.
  • Employed a simple instrumentation setup: a monochrome digital camera and a pulsed LED.
  • Achieved a time resolution of 80 μs, limited by camera electronics dead time.

Main Results:

  • MTPI-DDM successfully measured the diffusion of 147 nm polystyrene nanoparticles in water.
  • Extended the range of measurable short time lags by nearly two orders of magnitude compared to conventional continuous-wave DDM.
  • Eliminated motion blur, leading to enhanced DDM signal amplitudes.

Conclusions:

  • MTPI-DDM offers a substantial advancement in DDM capabilities for studying fast dynamics.
  • The technique provides a cost-effective and efficient method for high-resolution microscale dynamics analysis.
  • MTPI-DDM opens new avenues for investigating rapid processes in soft matter systems.