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Culture insert device with perfusable microchannels enhancesin vitroskin model development and barrier function

Dina Mikimoto1, Masahito Mori2, Akemi Toyoda3

  • 1The University of Tokyo, Tokyo, Japan.

Biofabrication
|April 3, 2024
PubMed
Summary

Researchers developed an innovative microfluidic device for advanced in vitro skin models. This system enhances skin structure development and barrier function assessment, crucial for cosmetic and drug testing.

Keywords:
TEERbarrier functionhuman skin equivalentmicrofluidic deviceperfusion culturepermeation test

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • In Vitro Skin Modeling
  • Microfluidics

Background:

  • Increasingly strict regulations on animal testing for cosmetics drive demand for in vitro skin research.
  • Advancements in in vitro skin models have progressed from simple bilayer structures to complex models incorporating hypodermis, vasculature, and appendages.
  • There is a need for sophisticated in vitro models that accurately mimic in vivo skin for research and testing.

Purpose of the Study:

  • To design and validate a novel microfluidic device for culturing in vitro skin models within standard 6-well plates.
  • To assess the barrier function and percutaneous absorption of the developed in vitro skin model.
  • To evaluate the impact of perfusion on the morphogenesis and vascularization of the in vitro skin model.

Main Methods:

  • Development of a microfluidic device featuring a reverse flange-shaped anchor for seamless integration into 6-well plates.
  • Culturing of in vitro skin models under both static and perfused conditions using the microfluidic device.
  • Assessment of epidermal morphogenesis, barrier function, and percutaneous penetration of caffeine via vascular absorption.

Main Results:

  • The microfluidic device enabled successful culturing and barrier function assessment of in vitro skin models without additional equipment.
  • Perfusion through vascular-like channels significantly improved epidermal morphogenesis compared to static culture.
  • The model allowed for effective evaluation of caffeine permeation and vascular absorption, key indicators for systemic drug exposure.

Conclusions:

  • The novel microfluidic device offers a versatile platform for advanced in vitro skin model development and testing.
  • Perfusion is a critical factor in enhancing the structural complexity and physiological relevance of in vitro skin models.
  • This technology facilitates robust assessment of skin barrier function and percutaneous absorption for cosmetic and pharmaceutical applications.