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    We developed a novel fly-scan strategy for coded ptychography (CP) microscopy, significantly increasing imaging speed. This method uses vibration motors and reduces artifacts, offering a cost-effective solution for high-throughput bio-imaging.

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    Area of Science:

    • Optical imaging
    • Microscopy
    • Computational imaging

    Background:

    • Coded ptychography (CP) enables lensless, high-resolution optical imaging.
    • Existing CP methods face throughput limitations due to inefficient scanning.
    • There is a need for faster, more cost-effective CP microscopy techniques.

    Purpose of the Study:

    • To introduce a novel 'fly-scan' strategy for high-throughput coded ptychographic microscopy.
    • To overcome the scanning inefficiencies and throughput limitations of conventional CP.
    • To develop a low-cost, DIY platform for advanced bio-imaging applications.

    Main Methods:

    • Implemented a 'fly-scan' scanning strategy using two eccentric rotating mass (ERM) vibration motors.
    • Developed a novel rolling-shutter distortion correction algorithm.
    • Constructed a low-cost, DIY-made prototype platform for coded ptychographic microscopy.

    Main Results:

    • The 'fly-scan' strategy eliminated scanning overhead and reduced periodic artifacts.
    • The rolling-shutter correction algorithm effectively addressed distortion effects.
    • Validated the platform's performance on various samples, including resolution targets, phase targets, and biospecimens.

    Conclusions:

    • The proposed 'fly-scan' method significantly enhances imaging throughput in coded ptychography.
    • The developed platform provides a cost-effective and efficient solution for high-throughput bio-imaging.
    • This approach holds promise for advancing lensless, high-resolution microscopy applications.