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Related Experiment Video

Updated: Jun 29, 2025

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
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Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids.

Adriano Bolondi1, Benjamin K Law2, Helene Kretzmer1

  • 1Department of Genome Regulation, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

Developmental Cell
|April 5, 2024
PubMed
Summary

Understanding how embryonic stem cells differentiate into neural and somitic cell types is key. This study reveals neuro-mesodermal progenitors (NMPs) mature over time, favoring neural lineage, and exhibit broad fate outcomes.

Keywords:
cell plasticityembryonic developmentlineage tracingmolecular recordingmorphogenesisneuro-mesodermal progenitor dynamicssingle-cell phylogeniesstem cell embryoids

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genomics

Background:

  • Embryogenesis involves complex cellular coordination for tissue development.
  • Understanding cell fate decisions in transient progenitor populations is challenging.

Purpose of the Study:

  • To build single-cell phylogenies of neuro-mesodermal progenitors (NMPs).
  • To analyze NMP behavior during differentiation into neural and somitic cell types.
  • To develop a framework for studying transient progenitor dynamics in development.

Main Methods:

  • Continuous Cas9-based molecular recording.
  • Mouse embryonic stem cell model of the embryonic trunk.
  • Single-cell phylogeny construction.

Main Results:

  • NMPs display subtle transcriptional signatures of recent differentiation.
  • Individual NMPs contribute to downstream lineages with broad fate distributions.
  • Axial progenitors intrinsically mature to favor the neural lineage over developmental time.

Conclusions:

  • Cellular decisions in embryogenesis are influenced by both environmental cues and intrinsic maturation.
  • A novel framework is presented for analyzing non-homeostatic dynamics of transient progenitor populations.
  • This research provides insights into how progenitor populations shape complex tissues during development.