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Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

A comprehensive guide to extract information from extracellular vesicles: a tutorial review towards novel analytical

Sabrina Romanò¹, Valeria Nele¹, Virginia Campani¹

¹Department of Pharmacy, University of Naples Federico II, Italy.

Analytica Chimica Acta

|April 5, 2024

Summary

Extracellular vesicles (EVs) are vital cell mediators carrying biochemical information. This review guides researchers on EV lysis protocols, crucial for developing new biomarkers for liquid biopsy and advancing measurement science.

Keywords:

Biomarkers Diagnostics Exosomes Extracellular vesicles Liquid biopsy Lysis

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Extraction of Extracellular Vesicles from Whole Tissue

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

Area of Science:

Biomedical Science
Molecular Biology
Analytical Chemistry

Background:

Extracellular vesicles (EVs) act as crucial cell mediators, carrying biomolecules from their parent cells.
EVs found in biofluids are promising circulating biomarkers for liquid biopsy due to their reflection of cell origin.
Clinical application of EVs faces challenges in protocol standardization, reagent cost, and analytical tool development.

Purpose of the Study:

To provide a practical guide for researchers developing novel analytical approaches for EVs.
To evaluate and discuss common EV lysis protocols, including their pros and cons.
To aid in the advancement of measurement science technology for EV analysis.

Main Methods:

Tutorial review of commonly used EV lysis protocols.
Evaluation of protocols based on experimental procedures, time, and equipment.
Consideration of significant EV applications and their mechanisms of action.

Main Results:

Discussion of advantages and disadvantages of various EV lysis protocols.
Identification of key challenges in EV biomarker detection and analysis.
Presentation of comprehensive tables for an overview of EV characterization.

Conclusions:

Standardization of lysis protocols is critical for reliable EV biomarker analysis.
Development of cost-effective reagents and sensitive analytical tools is essential for clinical EV applications.
This review offers practical insights to support researchers in advancing EV-based diagnostics.