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Updated: Jun 29, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
Published on: August 15, 2014
Selective-plane-activation structured illumination microscopy
Kenta Temma1,2,3, Ryosuke Oketani1,4, Toshiki Kubo1
1Department of Applied Physics, Osaka University, Osaka, Japan.
Abstract:
The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
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