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Updated: Jun 29, 2025

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
Published on: September 3, 2010
High-resolution multi-reflection time-of-flight mass spectrometer with atmospheric pressure interface
Shuxiong Yan1,2, Yi Ren1,3, Qi Huang1,4
1Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, China.
A new Atmospheric Pressure Interface Multi-Reflection Time-of-Flight Mass Spectrometry (API-MRTOF-MS) achieves high-resolution and rapid analysis. This advanced technique offers significant potential for diverse scientific applications.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Atmospheric Pressure Interface Multi-Reflection Time-of-Flight Mass Spectrometry (API-MRTOF-MS) offers potential for rapid, high-resolution analysis.
- Applications span chemistry, biology, environmental science, and medicine.
Purpose of the Study:
- To develop and demonstrate a high-resolution API-MRTOF-MS approach.
- To enhance mass resolution and analysis speed.
Main Methods:
- Ions are reflected using electrostatic mirrors and a folded flight path to increase distance.
- Extended flight path from meters to over 1 km.
- Utilized rapid response of Time-of-Flight Mass Spectrometry (TOF-MS) for analysis completion.
Main Results:
- Achieved a mass resolution of 116,050 (FWHM) for Cs+ ions.
- Analysis completed within 18 ms using atmospheric pressure electrospray ionization.
- Demonstrated over 50% ion transmission efficiency after 600 cycles.
Conclusions:
- The developed API-MRTOF-MS is suitable for high-resolution and rapid analysis.
- Confirms the technique's analytical performance for diverse scientific fields.
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