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Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

Yuanzhao Shen1, Chih-Tsung Yang2, Weiwei Li3

  • 1College of Veterinary Medicine, Institute of Comparative Medicine, Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. zhou_xin@126.com.

The Analyst
|April 8, 2024
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...