Compact organ-tissue electrophoresis system (CORES)

Area of Science:

• Biotechnology
• Laboratory Equipment
• Histology

Background:

• Electrophoretic tissue clearing is a long-standing laboratory technique.
• Standardized procedures and equipment for electrophoretic tissue clearing are lacking.
• Controlling heat, voltage, resistance, and circulation speed presents challenges for researchers.

Purpose of the Study:

• To develop a compact, user-friendly, and cost-effective organ electrophoresis system.
• To overcome the difficulties associated with traditional electrophoretic tissue clearing methods.
• To provide researchers with an easy, rapid, and inexpensive working opportunity.

Main Methods:

• Development of an integrated system including an electronic control unit, liquid tank, temperature control, and electrophoresis chamber.
• Implementation of software with sensors and a feedback principle for system stability (temperature and circulation).
• Utilization of platinum wires for electrodes to minimize corrosion and particle collection.

Main Results:

• The developed system, CORES, offers an all-in-one solution for electrophoretic tissue clearing.
• The system ensures efficient, rapid, and consistent tissue clearing.
• Continuous operation for 72 hours demonstrated system stability.

Conclusions:

• The CORES system provides a stable, efficient, and rapid solution for electrophoretic tissue clearing.
• The system's design addresses key challenges faced by researchers.
• Further development includes patent applications and trial studies for researcher introduction.