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Eukaryotic initiation factor 3a promotes the development of diffuse large B-cell lymphoma through regulating cell
Hongkun Sun1,2, Juanjuan Shang1, Xiao Liu2
1Department of Hematology, Shandong Provincial Hospital, Shandong University, 250021, Jinan, Shandong, China.
BMC Cancer
|April 8, 2024
Summary
High expression of eukaryotic initiation factor 3a (eIF3a) in diffuse large B-cell lymphoma (DLBCL) correlates with poor prognosis and promotes tumor growth. Targeting eIF3a may offer a new therapeutic strategy for DLBCL patients.
Area of Science:
- Oncology
- Molecular Biology
- Translational Medicine
Background:
- Diffuse large B-cell lymphoma (DLBCL) has a high relapse rate after standard treatment.
- Eukaryotic initiation factor 3a (eIF3a) is implicated in tumorigenesis and therapeutic response.
- Understanding eIF3a's role in DLBCL is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the biological role of eIF3a in DLBCL.
- To evaluate eIF3a as a prognostic biomarker in DLBCL.
- To explore eIF3a as a potential therapeutic target for DLBCL.
Main Methods:
- Utilized RNA-seq datasets from GEO to analyze eIF3a expression and prognosis.
- Assessed eIF3a protein levels via western blot and immunohistochemistry.
- Performed eIF3a knockdown in DLBCL cells to determine its biological functions and analyzed differentially expressed genes (DEGs).
Main Results:
- Elevated eIF3a expression in DLBCL patients correlated with worse prognosis.
- eIF3a knockdown suppressed DLBCL cell proliferation, reduced proliferation-related proteins, and increased apoptosis.
- Identified 114 DEGs linked to cell cycle and tumor immunity; eIF3a and DEG mutations affected chemosensitivity and signaling pathways.
Conclusions:
- First study to demonstrate high eIF3a expression and its prognostic significance in DLBCL.
- eIF3a promotes DLBCL development by regulating cell proliferation and apoptosis.
- eIF3a is linked to immune profiles and chemosensitivity, suggesting its potential as a prognostic biomarker and therapeutic target.
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