Related Experiment Video
Updated: May 11, 2026
08:26
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
13.7K
A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes
Chanida Chumchanchira1,2, Suwipa Ramphan2, Atchara Paemanee3
1PhD Degree Program in Biology (International Program), Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Scientific Reports
|April 9, 2024
Summary
Dengue virus (DENV) infection impacts host cells differently depending on the DENV type. Prohibitin 1 (PHB1) is downregulated by DENV 2 and DENV 4, but only DENV 2 interacts with PHB1, highlighting challenges in developing broad-spectrum dengue therapies.
Area of Science:
- Virology
- Proteomics
- Cell Biology
Background:
- Dengue virus (DENV) poses a significant global health challenge, with four distinct serotypes complicating vaccine and drug development.
- Previous in vitro proteomic studies on DENV infection primarily used DENV 2, limiting understanding of host-pathogen interactions across different DENV serotypes.
Purpose of the Study:
- To identify host proteins differentially regulated by two distinct DENV serotypes, DENV 2 and DENV 4.
- To investigate the role of prohibitin 1 (PHB1) in DENV infection and its interaction with viral proteins.
Main Methods:
- Comparative proteomic analysis using 2-dimensional gel electrophoresis to compare protein expression profiles in cells infected with DENV 2 and DENV 4.
- Western blotting and immunofluorescence assays to confirm protein expression changes and investigate protein-protein interactions.
Main Results:
- Proteomic analysis revealed only 11 differentially expressed proteins common to both DENV 2 and DENV 4 infection, with six coordinately regulated.
- Prohibitin 1 (PHB1) was consistently downregulated by both DENV serotypes.
- Overexpression of PHB1 enhanced DENV replication, and the DENV 2 envelope (E) protein directly interacted with PHB1, unlike the DENV 4 E protein.
Conclusions:
- Few host proteins are coordinately regulated by different DENV serotypes, posing challenges for identifying broad-spectrum therapeutic targets.
- The interaction between DENV E protein and PHB1 is serotype-specific, suggesting distinct mechanisms of host cell manipulation.
- Future research should investigate multiple DENV serotypes to better understand dengue pathogenesis and develop effective interventions.
Related Concept Videos
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:22Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...